A metal-organic framework-derived ruthenium-nitrogen-carbon nanozyme for versatile hydrogen sulfide and cystathionine γ-lyase activity assay.

In this study, a novel approach exploiting the interactions between hydrogen sulfide (HS) and ruthenium-nitrogen-carbon (Ru-N-C) nanozymes is presented, advancing HS and cystathionine γ-lyase (CSE) biosensing techniques. Utilizing the intrinsic peroxidase-like activity of Ru-N-C nanozymes and the noticeable inhibition effect caused by HS, an efficient, simple, and economical assay has been developed. This innovative method allows for the versatile real-time monitoring of HS from various sources, including specialized donors and native bacterial production. Furthermore, the assay has been applied to reveal the interactions within tumor cells and tissues ex vivo, providing a clearer and simpler view of CSE expression levels through an improved colorimetric method. This contribution enhances our understanding of the complex roles of HS metabolism and represents a significant advancement in the versatile application of nanozymes in biomedical research.