Description of Fusibacillus kribbianus gen. nov., sp. nov., a fusiform anaerobe isolated from pig feces.

Objective: The family Lachnospiraceae is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of Lachnospiraceae inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health.

Methods: The polyphasic taxonomic approach was used to characterize the isolate YH-rum2234. A detailed metabolic analysis was conducted to compare the novel isolate with related strains within the family Lachnospiraceae.

Results: A fusiform, obligately anaerobic, Gram-stain-negative bacterium, YH-rum2234, was isolated from pig feces. Analysis of the 16S rRNA gene sequence revealed that the similarities between the isolate and the familiarly interrelated strain Lientehia hominis KCTC 25345 was 94.3%. The average nucleotide identities and genome-to-genome distances of YH-rum2234 and its closely related strains were below 85.5% and 32.5%, respectively. The G + C content of the genomic DNA was 49.2 mol%. The main fatty acids were C, C, and C DMA. The major polar lipids were aminophospholipids. The cell wall did not contain the peptidoglycan meso-diaminopimelic acid.

Conclusion: Given the chemotaxonomic, phenotypic, and phylogenetic properties, YH-rum2234 (=KCTC 25710 = DSMZ 116041) represents a new genus and species in the family Lachnospiraceae. Fusibacillus kribbianus gen. nov., sp. nov. is the proposed name.