AI Article Synopsis
- TP10 is a special type of peptide that can help deliver drugs into cells and looks a lot like other peptides known for fighting germs.
- Researchers studied how TP10 kills leukemia cells and found that it breaks their membranes quickly, especially when there's a lot of it.
- Instead of causing a usual cell death called apoptosis, TP10 leads to different types of cell death called necrosis and necroptosis, which helps scientists understand why TP10 can be toxic.
Article Abstract
TP10, a classic cell-penetrating peptide, shows a high degree of similarity to AMPs in structure. Although TP10 has been widely used in drug delivery, the mechanism underlying its cytotoxicity is yet to be elucidated. Herein, we explored the cell-killing mechanism of TP10 against human leukemia Jurkat cells. TP10 induced necrosis in Jurkat cells via rapid disruption of cell membranes, particularly at high concentrations. Although mitochondria in Jurkat cells were damaged by TP10, mitochondria-mediated apoptosis did not occur, possibly due to intracellular ATP depletion. Necroptosis in TP10-treated Jurkat cells became an alternative route of apoptosis. Our results demonstrate that necrosis and necroptosis rather than apoptosis are involved in the cell-killing mechanism of TP10, which contributes to the understanding of its toxicity.
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| http://dx.doi.org/10.1016/j.biochi.2023.11.001 | DOI Listing |
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