Background: Over the past few years, there have been many reports on the abnormal expression and functional relevance of long non-coding RNAs (lncRNAs) in tumors. The role played by lncRNAs in epithelial ovarian carcinoma (EOC) remains poorly understood, however the goal of the present work was to study molecular mechanisms that underlie involvement of prostate androgen-regulated transcript 1 () lncRNA in EOC development.
Methods: A total of 25 tumor and 17 normal specimens were obtained from women undergoing surgery between 2015 and 2019 in the Second Affiliated Hospital, Nanjing Medical University. Expression levels for in EOC tissue and EOC cell lines were assessed using qRT-PCR. Assays for CCK-8, trans-well, colony forming and western blotting were used to investigate , miR-150-5p and ( proto-oncogene) for their invovement in EOC cell proliferation, migration and invasion. Luciferase reporter gene assay was also performed to investigate biological functions of , miR-150-5p and in EOC, and an animal xenograft model was employed to test tumorigenicity.
Results: expression was increased in EOC relative to normal cells and correlated with EOC cell proliferation, migration and invasion. can sponge miR-150, thereby inhibiting growth of EOC by targeting . The xenograft mouse model revealed that can regulate tumorigenesis .
Conclusions: The /miR-150/ axis is involved in EOC pathogenesis and could represent a new target to use in diagnosis and therapy.
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