AI Article Synopsis
- - This study focuses on the molecular analysis of Archamoebae, specifically analyzing the 18S rRNA gene sequence of Mastigamoeba aspera, the type species of its genus.
- - The research also includes characterization of the tubulin cytoskeleton of M. aspera using advanced microscopy techniques.
- - Findings led to a revision of the Mastigamoebidae family, establishing two new subfamilies based on differences in flagella structure and detailing unique features of the tubulin cytoskeleton in various Mastigamoeba species.
Article Abstract
In this study, we presented the results of our molecular phylogenetic analysis of Archamoebae using a newly obtained sequence of the 18S rRNA gene of Mastigamoeba aspera, the type species of the genus. In addition, we characterized the general organization of the tubulin cytoskeleton of M. aspera by immunofluorescent microscopy and TEM. Our findings allowed us to revise the family Mastigamoebidae and establish within it two subfamilies: Mastigamoebinae for the genus Mastigamoeba and Seraviniinae subfam. nov. for the genera Seravinia gen. nov., Paramastigamoeba gen. nov., Iodamoeba and Endolimax. The type genera Mastigamoeba and Seravinia are clearly distinguished by the structure of the basal apparatus of flagella. In addition, the tubulin cytoskeleton of several Mastigamoeba spp. contains a rim of microtubules around the nuclei.
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| http://dx.doi.org/10.1016/j.ejop.2023.126029 | DOI Listing |
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