Taxonomic descriptions of sp. nov., sp. nov. and sp. nov.

Six Gram-stain-positive, facultative anaerobic, nonmotile and rod-shaped strains, designated zg-Y50, zg-Y1362, zg-Y1379, zg-Y869, zg-629 and zg-Y636, were isolated from the intestinal contents of in Qinghai Province, PR China. Strains zg-Y50, zg-Y1379 and zg-629 exhibited the highest 16S rRNA gene sequence similarities of 99.2, 98.9 and 98.8 % to 9 H-4, JCM 14732 and TYLN1, respectively. Phylogenetic and phylogenomic analyses based on the 16S rRNA gene and genomic sequences, respectively, revealed that the six strains formed three distinct clades within the genus . The genome sizes of strains zg-Y50, zg-Y1379 and zg-629 were 3.1-3.7 Mb, with DNA G+C contents of 69.6-70.4 mol%. Average nucleotide identity and digital DNA-DNA hybridization values between each novel strain and available members of the genus were all below species thresholds. All novel strains contained MK-9 (H) as the major menaquinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the polar lipids. The predominant fatty acid of the six isolates was C 9. The cell-wall peptidoglycan contained ʟʟ-diaminopimelic acid as the diagnostic diamino acid. Based on the results from this polyphasic taxonomic study, three novel species in the genus are proposed, namely, sp. nov. (zg-Y50=GDMCC 1.2981=KCTC 49764), sp. nov. (zg-Y1379=GDMCC 1.2982=KCTC 49765) and sp. nov. (zg-629=CGMCC 1.17414=JCM 33888).