Cold seeps occur in continental margins worldwide and are deep-sea oases. Anaerobic oxidation of methane is an important microbial process in the cold seeps and plays an important role in regulating methane content. This study elucidates the diversity and potential activities of major microbial groups in dependent anaerobic methane oxidation and sulfate-dependent anaerobic methane oxidation processes and provides direct evidence for the occurrence of nitrate-/nitrite-dependent anaerobic methane oxidation (Nr-/N-DAMO) as a previously overlooked microbial methane sink in the hydrate-bearing sediments of the South China Sea. This study provides direct evidence for occurrence of Nr-/N-DAMO as an important methane sink in the deep-sea cold seeps.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10715046 | PMC |
http://dx.doi.org/10.1128/spectrum.02505-23 | DOI Listing |
ACS Omega
January 2025
Department of Biotechnology and Food Science, Durban University of Technology, Durban 4001, South Africa.
Anaerobic digestion is a crucial process in wastewater treatment, renowned for its sustainable biogas production capabilities and the simultaneous reduction of environmental pollution. However, dysregulation of vital biological processes and pathways can lead to reduced efficiency and suboptimal biogas output, which can be seen through low counts per million of sequences related to three critical control points for methane synthesis. Namely, tetrahydromethanopterin S-methyltransferase (MTR), methyl-coenzyme reductase M (MCR), and CoB/CoM heterodisulfide oxidoreductase (HDR) are the last reactions that must occur.
View Article and Find Full Text PDFBioresour Technol
January 2025
INSA Lyon, DEEP, UR7429 69621 Villeurbanne Cedex, France.
Biomethane production from biological methanation of CO is promising both for biogas upgrading and surplus renewable energy storage. One of the questions for process upscaling is the impact of oxygen (in the biogas or in the purified CO-rich off-gas) on the biological process. An adapted anaerobic thermophilic consortium was submitted to increasing amounts of oxygen in batch and continuous tests at partial pressures ranging from 0 to 50 mbar.
View Article and Find Full Text PDFBioresour Technol
January 2025
College of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Nanobubble water (NBW) or temperature-phased anaerobic digestion assisted by microbial electrolysis cell (MEC-TPAD) can promote sludge hydrolysis and methanogenesis. However, the role of the combined application of NBW and MEC-TPAD in terms of anaerobic performance and related microbial properties remains unclear. This study investigated the impact of Air-NBW on hydrolysis and methanogenesis of dewatered sludge MEC-TPAD.
View Article and Find Full Text PDFWater Res
January 2025
Laboratory of Biomass Bio-chemical Conversion, Guang Zhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address:
Propionate is a key intermediate in anaerobic digestion (AD) under low operational temperatures, which can destabilize the process. In this study, the supplementation of syntrophic cold-tolerant consortia and trace elements significantly improved the performance of psychrophilic (20 °C) reactor, increasing methane production to 91 % of mesophilic reactor levels and reducing propionate concentrations to less than 2 % of those in untreated psychrophilic reactors. Multi-omics analyses revealed that psychrophilic conditions downregulated the methylmalonyl-CoA and aceticlastic methanogenesis pathways.
View Article and Find Full Text PDFBioresour Technol
January 2025
Department of Frontier Science for Advanced Environment, Graduate School of Environmental Sciences, Tohoku University, 6-6-06 Aoba, Aramaki-Aza, Sendai, Miyagi 980-8579, Japan; Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki-Aza, Sendai, Miyagi 980-8579, Japan. Electronic address:
This study investigated the performance and phase-specific characteristics of mesophilic co-digestion of food waste (FW) with rice straw (RS) at different RS proportions (40 %, 60 %, and 80 %), as well as mono-digestion of RS. The system achieved optimal performance at 40 % RS content, with a methane yield of 383.8 mL/g-VS and cellulose removal efficiency exceeding 75 %.
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