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Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry. | LitMetric
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Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry.

Jinyuan Song Junlei Zhang Yongtao Ji Jianghui Tang Jianpeng Sheng Tingbo Liang Xueli Bai

STAR Protoc

Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address:

Published: December 2023

Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023)..

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630849PMC
http://dx.doi.org/10.1016/j.xpro.2023.102679DOI Listing

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