Evaluating bacterial-fungal interactions is important for understanding ecological functions in a natural habitat. Many studies have defined bacterial-fungal interactions according to changes in growth rates when co-cultivated. However, the current literature lacks detailed studies on phenotypic changes in single cells associated with transcriptomic profiles to understand the bacterial-fungal interactions. In our study, we measured the single-cell phenotypes of bacteria co-cultivated with fungi using Raman spectroscopy with its transcriptomic profiles and determined the consequence of these interactions in detail. This rapid and reliable phenotyping approach has the potential to provide new insights regarding bacterial-fungal interactions.
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http://dx.doi.org/10.1128/spectrum.00437-23 | DOI Listing |
Front Plant Sci
January 2025
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
Maize () is India's third-largest grain crop, serving as a primary food source for at least 30% of the population and sustaining 900 million impoverished people globally. The growing human population has led to an increasing demand for maize grains. However, maize cultivation faces significant challenges due to a variety of environmental factors, including both biotic and abiotic stresses.
View Article and Find Full Text PDFEnviron Res
January 2025
School of Ecology and Environment, Ningxia University, Yinchuan 750021, China. Electronic address:
Salinization processes profoundly impact soil quality and health, altering physical structure, chemical composition, and biological activity, particularly concerning soil microbial populations. Microbial communities play a pivotal role in maintaining soil ecosystem multifunctionality (EMF). Understanding the response of microbial communities to salinity stress is crucial for sustainable soil management and enhancing ecosystem resilience in arid and semi-arid regions.
View Article and Find Full Text PDFMycoses
January 2025
Unité de Parasitologie-Mycologie, Département de Prévention, Diagnostic et Traitement Des Infections, CHU Henri Mondor, Assistance Publique Des Hôpitaux de Paris (APHP), Creteil, France.
Background: The airways of patients with cystic fibrosis (pwCF) harbour complex fungal and bacterial microbiota involved in pulmonary exacerbations (PEx) and requiring antimicrobial treatment. Descriptive studies analysing bacterial and fungal microbiota concomitantly are scarce, especially using both culture and high-throughput-sequencing (HTS).
Objectives: We analysed bacterial-fungal microbiota and inter-kingdom correlations in two French CF centres according to clinical parameters and antimicrobial choices.
Proc Natl Acad Sci U S A
January 2025
Department of Plant Pathology, Key Laboratory of Plant Immunity, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Bacterial-fungal interaction (BFI) has significant implications for the health of host plants. While the diffusible antibiotic metabolite-mediated competition in BFI has been extensively characterized, the impact of intercellular contact remains largely elusive. Here, we demonstrate that the intercellular contact is a prevalent mode of interaction between beneficial soil bacteria and pathogenic filamentous fungi.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
Department of Plankton and Microbial Ecology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Stechlin, Germany; Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany. Electronic address:
Ecological impacts of tire wear particles (TWPs) on microbial communities and biogeochemical cycles in freshwater remain largely unknown. Here, we conducted a microcosm experiment to investigate interactions between the overlying water and sediment without and with TWPs addition in a rural vs. urban lake system.
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