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Physical Properties of Ultrafine Bubbles Generated Using a Generator System. | LitMetric

AI Article Synopsis

  • This study investigates the lifespan and ideal concentration of ultrafine bubbles (UFBs) for their use in biological applications, noting their promising properties.
  • The research utilized a generator to create UFBs and measured their size and concentration over time, finding a decrease in concentration of about 30% in the first two weeks but stability up to 6 months.
  • Results indicated that UFBs could be effectively generated for cell culture systems, as their concentration and size can be controlled and they significantly influence cell properties.

Article Abstract

Background/aim: Ultrafine bubbles (UFBs) have been extensively researched owing to their promising physical and biological properties. However, determining the lifespan or ideal concentration of UFBs for various biological events is challenging. This study aimed to determine the maximum concentration and longest lifespan of UFBs and to verify the validity of UFBs for assessing cell properties.

Materials And Methods: A generator system (HMB-H0150+P001, TOSSLEC Corporation Limited, Kyoto, Japan) generated UFBs using various gases. The size and concentration of UFBs in ultrapure water and cell culture medium were measured through a nanoparticle tracking analysis method.

Results: The UFB concentration increased when the generator operated in a time dependent manner. The mean size of UFBs was approximately 120 nm. In the UFB lifespan, the concentration decreased by approximately 30% within the first two weeks of generation and was stable for up to 6 months. The UFB size increased by approximately 20% within the first two weeks of generation and demonstrated minor changes until the 6th month. The number of cells differed significantly with various concentrations of nitrogen gas UFBs.

Conclusion: The generator system can generate UFBs with multiple concentrations within a suitable temperature. Consequently, the solution containing UFBs could be widely acceptable in cell culture systems.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621414PMC
http://dx.doi.org/10.21873/invivo.13363DOI Listing

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