Background: Ecosystems in protected areas are richer in animal and plant species diversity ("biodiversity hotspots") due to more stringent conservation conditions. Of particular importance is scientific research and monitoring of this diversity in such areas. The aim of this study is to describe a set of data on Araneae occurrence in two protected areas: Mordovia State Nature Reserve and National Park "Smolny".
New Information: About 29,000 individuals are identified to the species level. In total, 342 species were recorded for both PAs. The greatest species diversity was recorded in the families Linyphiidae (109 species; 32%), Lycosidae (38 species; 11%) Gnaphosidae (28 species; 8%), Araneidae (25 species; 7%), Salticidae (24 species; 7%), Thomisidae (23 species; 7%) and Theridiidae (22 species; 6% from total species diversity). The five species most abundant in the lower stratum (litter and moss layer) of biocenoses were , , , and and the five species most abundant in the vegetative stratum (herb, shrub and tree stems and canopy) were , , , and . The dataset contains information on the occurrence of seven rare species (, , , , , and ), three species (, , ) that entered here from the steppe zone and two synanthropic species (, ).
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612110 | PMC |
http://dx.doi.org/10.3897/BDJ.11.e105979 | DOI Listing |
Methods Mol Biol
January 2025
School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Yeast two-hybrid library screening enables the discovery of novel protein-protein interactions. Identifying cytosolic host proteins targeted by host-translocated Phytophthora effector proteins relies on the mRNA amount, quality, and composition used to prepare the yeast two-hybrid cDNA library. Here we describe the steps required for the preparation of a Pinus radiata cDNA library optimized for Phytophthora effector target screening in yeast.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Horticultural Crops Disease and Pest Management Research Unit, United States Department of Agriculture-Agricultural Research Service, Corvallis, OR, USA.
Pathogens have evolved effector proteins to suppress host immunity and facilitate plant infections. RxLR effectors are small, secreted effector proteins with conserved RxLR and dEER amino acid motifs at the N terminus and highly variable C termini and are commonly found in oomycete species. We provide computational approaches to annotate RxLR candidate effector genes in a genome assembly in FASTA format with an available GFF file.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Here, we present a protocol for the isolation and detection of Phytophthora oospores directly from soil samples. Our method incorporates a novel technique for isolating Phytophthora oospores using filter pouches and an improved DNA extraction procedure specifically designed for oospores. While we have primarily developed this protocol for detecting P.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Sanya Institute of Nanjing Agricultural University, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
The establishment of reliable and efficient systems for genome editing in Phytophthora is very important for studying gene functions. Here, step-by-step methods for CRISPR/Cas9-based gene knockout and in situ complementation for Phytophthora sojae are presented. These steps include the sgRNA design, Cas9-sgRNA plasmid construction, homologous replacement, complementation vector construction, P.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Cell and Molecular Sciences Department, The James Hutton Institute, Dundee, UK.
At the core of assays to understand the role(s) of specific genes is the ability to stably transfer genes into Phytophthora through transformation. A key method for achieving this has been based on polyethylene glycol (PEG)/CaCl transformation of protoplasts, but efficiency has often been low. Improving transformation efficiency is necessary for many applications, such as gene knockouts.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!