Development of a Real-Time PCR Assay for the Detection of the Phlebovirus Fermo Virus.

Viruses

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "B. Ubertini", 25124 Brescia, Italy.

Published: October 2023

AI Article Synopsis

  • Fermo virus has been increasingly found in sand flies in northern Italy, but its natural transmission cycle is not well understood.
  • While there is evidence that Fermo virus can infect vertebrates, it has only been detected in sand flies, and no direct cases have been reported in animals or humans.
  • A new real-time PCR method has been developed for detecting Fermo virus, which offers more sensitive detection in insects and has the potential to identify the virus in vertebrates, aiding in the study of its epidemiology and potential risks to health.

Article Abstract

Fermo virus is a that is increasingly reported in sand flies from northern Italy. The natural cycle is not fully understood, but the virus has been detected by direct methods only in sand flies. Although there is serological evidence that it can infect vertebrates, the virus has not been directly detected in animals or humans. Here, we have developed and reported a specific real-time PCR for Fermo virus. The availability of the described method will be useful to characterize the epidemiology of the FERV, ensuring, compared to previously available protocols, a more sensitive detection in insects and the possible detection in vertebrates to evaluate the presence of reservoirs and the pathogenic potential of the virus in humans or animals.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612048PMC
http://dx.doi.org/10.3390/v15102082DOI Listing

Publication Analysis

Top Keywords

fermo virus
12
real-time pcr
8
sand flies
8
virus
6
development real-time
4
pcr assay
4
assay detection
4
detection phlebovirus
4
phlebovirus fermo
4
virus fermo
4

Similar Publications

In Italy, as in the rest of the world, government restrictions aimed at containing the spread of COVID-19 primarily imposed limitations on social relationships and personal behavior. This situation significantly affected the management of chronic illnesses, including type 2 diabetes (T2D). The objective was to evaluate the perceptions of patients with T2D regarding the quality of care received during the COVID-19 pandemic and the impact on dietary and physical activity behaviors.

View Article and Find Full Text PDF

Behind the scenes: metagenomic analysis of bacterial communities in sustainable depilation of sheepskin.

J Appl Microbiol

November 2024

New Zealand Leather and Shoe Research Association, PO Box 8094, Hokowhitu, Palmerston North 4446, New Zealand.

Aim: The leather industry is embracing eco-friendly technologies for both regulatory compliance and sustainable growth. While enzymatic depilation provides a greener alternative to traditional beamhouse methods, its complexity often leads to higher costs. To address this, we examined the performance of sheepskins' native bacterial flora in acetic acid conditions with low-environmental impact.

View Article and Find Full Text PDF
Article Synopsis
  • The study focused on the perioperative outcomes of pancreatic cancer surgeries during the COVID-19 pandemic, comparing data from 2019 and 2020.
  • Researchers analyzed 1815 patients, finding no significant difference in cancer stages between the two years, but noted a decrease in neoadjuvant chemotherapy use and an increase in minimally invasive procedures in 2020.
  • Despite some changes in treatment protocols and a rise in medical complications during surgery in 2020, the overall outcome for patients undergoing surgery for pancreatic cancer remained largely unaffected.
View Article and Find Full Text PDF

Epidemiological and clinical analysis of exposure-related factors in non-melanoma skin cancer: A retrospective cohort study.

Environ Res

April 2024

Dermatology Unit, Policlinico Tor Vergata, System Medicine Department, University of Tor Vergata, Rome, Italy, via Montpellier 1, 00133, Rome, Italy. Electronic address:

Background: The incidence of non-melanoma skin cancers (NMSCs) increased over last decades, probably due to environmental concerns or to the increase of frail patients with age related comorbidities. Currently, the relationship of increasing global skin cancer rates with increased ultraviolet radiations (UVRs) resulting from stratospheric ozone depletion, global warming, and air pollution from fossil-fuel combustion.

Aims: We conducted a retrospective epidemiological study including 546 NMSC patients managed at the Dermatology Unit of the Tor Vergata Hospital to highlight different trends of sun exposure or different comorbidities.

View Article and Find Full Text PDF
Article Synopsis
  • Phleboviruses are divided into two main groups: the sandfly fever group (spread by sandflies and mosquitoes) and the Uukuniemi group (spread by ticks).
  • The study focused on identifying sandfly species and detecting sandfly-borne viruses (SBVs) in Iran, using PCR techniques.
  • Researchers identified five positive specimens for phleboviruses, shedding light on the molecular epidemiology of Tehran virus, sandfly fever Sicilian virus, and Karimabad virus in the region.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!