AI Article Synopsis
- 6-nitrodopamine is identified as the major catecholamine released from rat isolated ventricles, demonstrating significantly higher potency compared to dopamine and noradrenaline.
- The study utilizes LC-MS/MS to quantify catecholamines and measures heart function through left ventricular developed pressure (LVDP), revealing that even minimal doses of 6-nitrodopamine can effectively increase heart contractility.
- The research highlights that 6-nitrodopamine release can be inhibited by L-NAME treatment, and its positive effects on LVDP can be blocked by atenolol, confirming its role as a key regulator of cardiac function.
Article Abstract
Background: 6-nitrodopamine released from rat isolated atria exerts positive chronotropic action, being more potent than noradrenaline, adrenaline, and dopamine. Here, we determined whether 6-nitrodopamine is released from rat isolated ventricles (RIV) and modulates heart inotropism.
Methods: Catecholamines released from RIV were quantified by LC-MS/MS and their effects on heart inotropism were evaluated by measuring left ventricular developed pressure (LVDP) in Langendorff's preparation.
Results: 6-nitrodopamine was the major released catecholamine from RIV. Incubation with L-NAME (100 µM), but not with tetrodotoxin (1 µM), caused a significant reduction in 6-nitrodopamine basal release. 6-nitrodopamine release was significantly reduced in ventricles obtained from L-NAME chronically treated animals. 6-nitrodopamine (0.01 pmol) caused significant increases in LVDP and dP/dt, whereas dopamine and noradrenaline required 10 pmol, and adrenaline required 100 pmol, to induce similar increases in LVDP and dP/dt. The infusion of atenolol (10 nM) reduced basal LVDP and blocked the increases in LVDP induced by 6-ND (0.01 pmol), without affecting the increases in LVDP induced by 10 nmol of dopamine and noradrenaline and that induced by adrenaline (100 nmol).
Conclusions: 6-nitrodopamine is the major catecholamine released from rat isolated ventricles. It is 1000 times more potent than dopamine and noradrenaline and is selectively blocked by atenolol, indicating that 6-ND is a main regulator of heart inotropism.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607994 | PMC |
| http://dx.doi.org/10.3390/life13102012 | DOI Listing |
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