Hepatitis E virus genotype 3 (HEV-3) is a food-borne pathogen causative of hepatitis E infections in humans. In Europe, HEV-3 is mainly transmitted through the consumption of raw or undercooked pork. In order to determine the effectiveness of control measures that can be taken in the industry or by the consumer, it is pivotal to determine the infectivity of HEV present in pork products after thermal food-processing steps. First, we implemented a method for the detection of infectious HEV-3c and HEV-3e in a cell culture medium and in extracts from inoculated pork products. Next, we investigated the effect of the thermal inactivation of HEV by mimicking food-processing steps specific for dried sausage and liver homogenate matrices. After four weeks, HEV-inoculated dried sausage subjected to 21 °C or lower temperatures was still infectious. For the liver homogenate, the highest HEV-3c/e inactivation of the conditions tested was observed at 71 °C for five min or longer. Finally, our method was able to successfully detect and estimate viral loads of infectious HEV in naturally infected pig livers. Our data provide a basis for the future use of the quantitative microbial risk assessment of infectious HEV in pork products that are subjected to thermal food processing steps.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10609450 | PMC |
| http://dx.doi.org/10.3390/microorganisms11102451 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An Investigation of the Status of Commercial Meat Analogs and Their Ingredients: Worldwide and South Korea.
Food Sci Anim Resour
January 2025
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Meat analogs are a burgeoning industry, with plant-based meat analogs, insect-based meat analogs, algae-based meat analogs, mycoprotein-based meat analogs, and cell-based meat analogs. However, despite the industry's growth potential, market expansion faces hurdles due to taste and quality disparities compared to traditional meats. The composition and characteristics of meat analogs currently available in the market are analyzed in this study to inform the development of future products in this sector.
View Article and Find Full Text PDFApplication of Animal Resources into the Maillard Reaction Model System to Improve Meat Flavor.
Food Sci Anim Resour
January 2025
Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Simulating meat flavor via Maillard reaction model systems that contain a mixture of amino acids and reducing sugars is an effective approach to understanding the reaction mechanism of the flavor precursors. Notably, animal resources such as fish, beef, chicken, pork hydrolysates, and fats are excellent precursors in promoting favorable meaty and roasted flavors and umami tastes of Maillard reaction products. The experimental conditions and related factors of the model systems for sensory enhancements, debittering, and off-flavor reduction with meat and by-products are summarized in this review.
View Article and Find Full Text PDFQuercetin Supplementation Reduces Oxidative Stress in the Testes of Wistar Rats Fed a High-Fat Diet.
Am J Reprod Immunol
January 2025
Laboratory of Molecular Genetics and Experimentation in Animal Reproduction, University of Western São Paulo (Unoeste), Presidente Prudente, São Paulo, Brazil.
Problem: A high-fat diet (HFD) predisposes animals to glucose intolerance, dyslipidemia and testicular oxidative stress, and impairs sperm production in rats. Quercetin is a flavonoid with antioxidant, anti-inflammatory, and lipolytic actions and is a potential supplement to combat the oxidative stress caused by HFD and its harmful effects on reproduction. This study evaluated the effects of quercetin supplementation at doses of 10 and 20 mg/day on reproductive parameters and testicular oxidative stress in Wistar rats fed a diet rich in pork fat and fructose.
View Article and Find Full Text PDFFirst report of in a wolf in Italy: An increasing health concern?
Food Waterborne Parasitol
March 2025
Unit of Foodborne and Neglected Parasitic Diseases, Department of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
is a zoonotic nematode parasite of worldwide distribution. It is present in Europe with important foci, particularly in Eastern countries and Spain. This species is generally associated with a domestic cycle that involves primarily pigs.
View Article and Find Full Text PDFFabrication of alginate dialdehyde-gelatin crosslinked hydrogels incorporated with cinnamaldehyde nanoparticles for meat preservation.
Int J Biol Macromol
January 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410007, PR China. Electronic address:
Meat spoilage caused by Salmonella Typhimurium (S. typhimurium) contamination has become a major concern worldwide. Herein, the antibacterial mechanisms of cinnamaldehyde (CIN) against S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!