Effect of on Subcutaneous Preadipocytes Proliferation in Goats.

The presence or absence of subcutaneous adipose accumulation will affect the energy storage, insulation resistance and metabolism of animals. Proliferation and differentiation of preadipocytes play a significant role in lipid deposition. The objective of this study was to clone the goat gene and investigate its potential functions on goat subcutaneous preadipocytes' proliferation by gaining or losing function in vitro. The goat gene was cloned by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and bioinformatics analysis was performed. The expression of the gene in the different goat tissues and adipocytes at different differentiation stages was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the cloned sequence of goat gene is 728 bp and the CDS region is 357 bp, encoding 118 amino acids. protein is located in nucleus, cytoplasm, mitochondria and extracellular matrix. Tissue-expression profiles revealed that expressed in all of the examined tissues. In visceral tissues, the highest expression level was found in lung ( < 0.01); in muscle tissues, the highest expression level was found in the longissimus dorsi ( < 0.01) and in adipose tissues, the highest expression level was found in subcutaneous adipose ( <0.01). Compared with those cells before differentiation, expression levels upregulated at 48 h ( < 0.01), 72 h ( < 0.01), 120 h ( < 0.01) and downregulated at 96 h ( < 0.01). Furthermore, the results of crystal violet staining and semi-quantitative assay showed that transfection with 1 μg expression plasmid reduced the cell numbers in vitro. Meanwhile, the expression of was significantly decreased. A similar consequence happened after interfering with expression. However, plasmid transfected with 2 μg pEGFPN1- increased the number of cells in vitro. These results suggest that is involved in the proliferation of goat subcutaneous preadipocytes.