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Engineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize. | LitMetric

Engineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize.

Insects

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China.

Published: October 2023

AI Article Synopsis

  • - Genetic engineering enhances crops' traits like insect resistance, but concerns about GM proteins in food have arisen among the public.
  • - A new Cre/P-based strategy allows for targeted expression of transgenes in specific plant tissues of maize, keeping them off in edible parts while enabling them in green tissues.
  • - Transgenic maize hybrids demonstrated high resistance to common pests, achieving over 73.6% mortality rates, highlighting the effectiveness and potential safety of this genetic engineering method.

Article Abstract

Genetic engineering technology offers opportunities to improve many important agronomic traits in crops, including insect-resistance. However, genetically modified (GM) exogenous proteins in edible tissues of transgenic crops has become an issue of intense public concern. To advance the application of GM techniques in maize, a Cre/P-based strategy was developed for manipulating the transgenes in green tissues while locking them in non-green tissues. In the strategy, the site-specific excision can be used to switch on or off the expression of transgenes at specific tissues. In this work, two basic transgenic maize, named KEY, carrying the gene, and LOCK, containing the gene with a blocked element, were obtained based on their separate fusion gene cassettes. The expression level and concentration of Vip3A were observed with a high specific accumulation in the green tissues (leaf and stem), and only a small amount was observed in the root and kernel tissues in the KEY × LOCK hybrids. The insect resistance of transgenic maize against two common lepidopteran pests, and , was assessed in the laboratory and field. The results indicate that the hybrids possessed high resistance levels against the two pests, with mortality rates above 73.6% and damage scales below 2.4 compared with the control group. Our results suggest that the Cre/P-mediated genetic engineering approach has a competitive advantage in GM maize. Overall, the findings from this study are significant for providing a feasible strategy for transgenes avoiding expression in edible parts and exploring novel techniques toward the biosafety of GM plants.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10607264PMC
http://dx.doi.org/10.3390/insects14100803DOI Listing

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