Polymyxin B and fusidic acid, a novel potent synergistic combination against and isolates with polymyxin B resistance.

Front Microbiol

Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Published: October 2023

The increasing prevalence of multidrug-resistant (MDR) Gram-negative bacteria and comparatively limited options of antibiotics pose a major threat to public health worldwide. Polymyxin B is the last resort against extensively resistant Gram-negative bacterial infections. However, a large number of Gram-negative bacteria exhibited high-level resistance to Polymyxin B, bringing challenges for antimicrobial chemotherapy. Combination therapies using polymyxins and other antibiotics are recommended to treat multidrug-resistant pathogens. In this study, we selected Gram-negative bacterial strains, including and , to explore whether fusidic acid and polymyxin B have a synergistic killing effect. Through broth microdilution, we observed that minimum inhibitory concentrations (MICs) against polymyxin B in the isolates tested were significantly reduced by the addition of fusidic acid. Notably, chequerboard analysis indicated a synergistic effect between polymyxin B and fusidic acid. In addition, subsequent time-kill experiments showed that the combination of polymyxin B and fusidic acid was more effective than a single drug in killing bacteria. Finally, our investigation utilizing the murine model revealed a higher survival rate in the combination therapy group compared to the monotherapy group. Our research findings provide evidence of the synergistic effect between polymyxin B and fusidic acid. Fusidic acid was shown to increase the sensitivity of multi-drug resistant and to polymyxin B, thereby enhancing its bactericidal activity. This study provides new insights into a potential strategy for overcoming polymyxin B resistance, however, further investigations are required to evaluate their feasibility in real clinical settings.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598591PMC
http://dx.doi.org/10.3389/fmicb.2023.1220683DOI Listing

Publication Analysis

Top Keywords

fusidic acid
28
polymyxin fusidic
16
polymyxin
11
polymyxin resistance
8
gram-negative bacteria
8
gram-negative bacterial
8
synergistic polymyxin
8
acid
7
fusidic
6
acid novel
4

Similar Publications

Clinical characteristics and genomic changes of recurrent Methicillin-Resistant Staphylococcus aureus bacteremia.

J Microbiol Immunol Infect

November 2024

Division of Pediatric Infectious Diseases, Chang Gung Memorial Hospital, 333, Taoyuan, Taiwan; Molecular Infectious Diseases Research Center, Chang Gung Memorial Hospital, 333, Taoyuan, Taiwan; Chang Gung University School of Medicine, 333 Taoyuan, Taiwan. Electronic address:

Background: Recurrent or persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia presents significant clinical challenges. Comprehensive genomic-scale studies on the genetic changes in MRSA that correspond to refractory bacteremia are lacking.

Method: From 2011 to 2019, MRSA blood isolates were collected from patients with persistent or recurrent bacteremia at a teaching hospital in southern Taiwan.

View Article and Find Full Text PDF
Article Synopsis
  • Listeria innocua is usually harmless but can become a threat for those with weak immune systems; it serves as a vital marker for food safety and sanitation effectiveness.
  • A study collected 39 L. innocua strains from foods like beef, fish, milk, and cheese in Turkey, identifying several virulence genes and high levels of antibiotic resistance, particularly to fusidic acid and oxacillin.
  • Results indicate that all isolates were resistant to at least one antibiotic, with most being multidrug resistant, emphasizing the need for better monitoring of L. innocua for potential risks to human and animal health.
View Article and Find Full Text PDF

Pregnane X receptor (PXR) is a xenobiotic-sensing nuclear receptor with a well-established role in regulating drug metabolism and clearance. Recent studies have shown that PXR is involved in cell proliferation, apoptosis, immune response, and energy homeostasis. It is important to identify compounds that may modulate PXR activity to prevent drug-drug interactions, distinguish chemicals which could potentially generate toxicity, and identify compounds for further development towards therapeutic usage.

View Article and Find Full Text PDF

Constructed wetlands (CWs) have been proven to effectively remove antibiotic resistance genes (ARGs) at different experimental scales; however, there is still a lack of researches on the removal and monitoring of ARGs during the actual operation of full-scale CWs. To fill this gap, this study selected the Annan constructed wetland in Beijing as a case study and utilized quantitative sequencing, metagenomic analysis, and other technical methods to determine characteristics of ARGs in CWs during different operating periods. Furthermore, we analysed the overall removal characteristics of ARGs in the CW during different operating periods and differences of ARG distribution in three media.

View Article and Find Full Text PDF

[Special lesion on back of the hand].

Ned Tijdschr Geneeskd

October 2024

Rijnstate, afd. Dermatologie, Arnhem.

A 74-year-old man, previously healthy, presented at the Dermatology department with an asymptomatic hand lesion persisting for two months. The general practitioner initially treated the lesion with betamethasone and fusidic acid cream. Examination revealed a 3 cm livid plaque with central crusts.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!