AI Article Synopsis
- The fission yeast shares genomic manipulation ease with budding yeast, despite their biological differences.
- Most tools used in budding yeast have corresponding versions for fission yeast that respect the specific characteristics of each.
- The text discusses a powerful method for site-directed mutagenesis, adapted for fission yeast, enabling quick, efficient, and marker-free modifications of nonessential genes.
Article Abstract
The fission yeast, like budding yeast, offer an easy manipulation of their genome, despite their distinct biology. Most tools available in budding yeast are also available in fission yeast in versions taking into account the features of each organism. The is a powerful approach, initially developed in , for site-directed mutagenesis. Here, we present an adaptation of the approach to manipulation and demonstrate its applicability for a rapid, marker-free and efficient site-directed mutagenesis and N-terminal tagging of nonessential genes in fission yeast.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594131 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000997 | DOI Listing |
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