Unlabelled: Numerous components of the transcription machinery, including RNA polymerase II (Pol II), accumulate in regions of high local concentration known as clusters, which are thought to facilitate transcription. Using the histone locus of nurse cells as a model, we find that Pol II forms long-lived, transcriptionally poised clusters distinct from liquid droplets, which contain unbound and paused Pol II. Depletion of the Integrator complex endonuclease module, but not its phosphatase module or Pol II pausing factors disperses these Pol II clusters. Consequently, histone transcription fails to reach peak levels during S-phase and aberrantly continues throughout the cell cycle. We propose that Pol II clustering is a regulatory step occurring near promoters that limits rapid gene activation to defined times.
One Sentence Summary: Using the histone locus as a model, we show that clustered RNA polymerase II is poised for synchronous activation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592978 | PMC |
| http://dx.doi.org/10.1101/2023.10.07.561364 | DOI Listing |
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