AI Article Synopsis
- Tongue dorsum swabbing is a promising alternative to traditional sputum collection for TB testing, showing high sensitivity and specificity with the Xpert MTB/RIF Ultra test.
- A study with 321 participants in South Africa tested both Xpert Ultra and Molbio Truenat MTB Ultima using tongue swabs and found that Xpert Ultra had a sensitivity of 75.4% and MTB Ultima had 71.6%.
- The findings indicate that tongue swabs allow for easier sample collection and maintain accurate results, making them suitable for high-throughput TB testing.
Article Abstract
Tongue dorsum swabbing is a potential alternative to sputum collection for tuberculosis (TB) testing. Previous studies showed that Cepheid Xpert MTB/RIF Ultra (Xpert Ultra) can detect (MTB) DNA in tongue swabs stored in buffer, with 72% sensitivity and 100% specificity relative to a sputum microbiological reference standard (sputum MRS). The present study evaluated a more convenient sample collection protocol (dry swab storage), combined with streamlined sample processing protocols, for side-by-side analysis using two commercial TB diagnostic tests: Xpert Ultra and Molbio Truenat MTB Ultima (MTB Ultima). Copan FLOQSwabs were self-collected, or collected by study workers, from 321 participants in Western Cape, South Africa. All participants had symptoms suggestive of TB, and 245 of them had sputum MRS-confirmed TB (by sputum culture and/or Xpert Ultra). One tongue swab per participant was tested on Xpert Ultra and another tongue swab was tested with MTB Ultima. Xpert Ultra was 75.4% sensitive and 100% specific, and MTB Ultima was 71.6% sensitive and 96.9% specific, relative to sputum MRS. When sample lysates that were false-negative by MTB Ultima were frozen, thawed, and re-tested, MTB Ultima sensitivity rose to 79.1%. Both tests were more sensitive with swabs from participants with higher sputum Xpert semi-quantitative results. The protocol for Xpert Ultra enabled fast and easy testing of dry-stored swabs with no loss of accuracy relative to previous methods. MTB Ultima testing of dry-stored swabs exhibited comparable performance to Xpert Ultra. These results further support tongue swabs as easy-to-collect samples for high-throughput TB testing.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593025 | PMC |
| http://dx.doi.org/10.1101/2023.10.10.23296833 | DOI Listing |
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