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Phenylalanine-Arginine-β-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on . | LitMetric

Phenylalanine-Arginine-β-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on .

Photobiomodul Photomed Laser Surg

Department of Infectious Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, China.

Published: October 2023

AI Article Synopsis

  • * It was found that the combination of MB with PAβN significantly improved bacterial killing compared to MB alone, with the best results coming from administering PAβN before MB.
  • * The results suggest that using PAβN enhances the antibacterial effect of MB-aPDI by possibly targeting bacterial efflux pumps, particularly when PAβN is applied first.

Article Abstract

To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-β-naphthylamide (PAβN) against . bacterial suspension was incubated with MB for different times (5-240 min), and then, 10 J/cm red light was irradiated. The efflux pump inhibitor (EPI) PAβN (10-100 μg/mL) was combined with MB (1-20 μM) in different sequences (PAβN-first, PAβN+MB, PAβN-after). Colony-forming units were then determined by serial dilution. Using MB 10 μM plus 10 J/cm, the killing effect of MB-aPDI on increased first and then decreased with longer incubation time. The killing effect of MB+PAβN-aPDI on was better than that of MB-aPDI ( < 0.05) by up to 2 logs. PAβN-first had the best killing effect, whereas PAβN-after had the worst killing effect. The killing effect increased with PAβN concentration and at 100 μg/mL reached 5.1 logs. The EPI PAβN enhanced the bactericidal effect of MB-aPDI on , especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10615054PMC
http://dx.doi.org/10.1089/photob.2023.0005DOI Listing

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