Manganese peroxidase (MnP) offers significant potential in various environmental and industrial applications; however, its reliance on Mn ions for electron shuttling limits its use in Mn-deficient systems. Herein, a novel approach is presented to address this limitation by co-immobilizing MnP and Mn in silica gels. These gels were synthesized following the standard sol-gel method and found to effectively immobilize Mn ions, primarily through electrostatic interactions. The MnP co-immobilized with Mn ions in the silica gel exhibited 4-5 times higher activity than the MnP immobilized alone in activity assays, and generated Mn within the gel, indicating the immobilized Mn ions remain capable of shuttling electrons to the co-immobilized MnP. In decolorization tests with two organic dyes, the co-immobilized system also outperformed the MnP immobilized without Mn ions, resulting in 2-4 times higher dye removals. This study will enable a broader application of MnP enzymes in sustainable environmental remediation and industrial catalysis.
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| http://dx.doi.org/10.1016/j.biortech.2023.129897 | DOI Listing |
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