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Design and development of a lanthanide-labeled immunochromatographic strip for simultaneous detection of morphine, methamphetamine and ketamine in hair. | LitMetric

AI Article Synopsis

  • Colloidal gold immunoassays are popular for drug detection but struggle with accuracy and sensitivity, especially for detecting drugs in hair samples.
  • To address these issues, researchers developed a new immunochromatographic test strip utilizing fluorescent nanoparticles, allowing for simultaneous screening of multiple drugs with significantly improved detection limits (100-1000 times lower than traditional methods).
  • The new method demonstrated high stability, low cross-reactivity among tested drugs, and produced results comparable to established LC-MS techniques, making it a promising tool for forensic drug screening.

Article Abstract

Colloidal gold immunoassay is the most widely used method in the field of drug detection. However, this method often has poor quantitative identification ability and low analytical sensitivity, which is not suitable for the analysis of hair poisoning ingredients. In order to solve these limitations, we developed an immunochromatographic test strip for simultaneously screening multiple drugs in this study. This hand-held test strip used fluorescent nanoparticles loaded with lanthanide chelates as the signal carrier of fluorescence reading, and conducted quantitative testing of various drugs based on the competitive immune reaction between the analyte and antigen. Under the optimal conditions, the competition curves of morphine (MOP), methamphetamine (MET) and ketamine (KET) were obtained on a single band. The detection limit (LOD) of this analytical method was 100-1000 times lower than that of colloidal gold test strips. The detection limits of MOP, MET and KET were 0.06 ng mL, 0.1 ng mL and 1.0 ng mL, respectively. No cross-reaction was observed when morphine, methamphetamine and ketamine were tested simultaneously with this method. And 184 hair samples were tested simultaneously, and the detected amount was very close to the results of LC-MS. The immunochromatographic strip showed good stability in repeated tests, and the coefficient of variation was less than 15%. Fluorescence immunochromatography strips and handheld strip readers have the characteristics of portability, speed, ease of operation and high sensitivity, and may become powerful tools for screening drug abuse in hair in forensic medicine.

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Source
http://dx.doi.org/10.1039/d3ay01280hDOI Listing

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