Unravelling Species Diversity and Pathogenicity of spp. Associated with Soybean Leaf and Root in Heilongjiang Province, China.

Soybean ( L.) holds significant global importance and is extensively cultivated in Heilongjiang Province, China. Soybean can be infected by species, causing root rot, seed decay, stem rot, and leaf blight. In 2021 to 2022, a field survey of soybean diseases was carried out in 11 regions of Heilongjiang Province, and 186 soybean leaves with leaf blight symptoms and 123 soybean roots with root rot symptoms were collected. Unexpectedly, a considerable number of isolates were obtained not only from root samples but also from leaf samples. A total of 584 isolates (416 from leaves and 168 from roots) were obtained and identified as 18 species based on morphological features and multilocus phylogenetic analyses with and sequences. and sp. 1 in FOSC were the dominant species within soybean leaf and root samples, respectively. Pathogenicity tests were conducted for all isolates on both soybean leaves and roots. Results showed that , , , , , , and were pathogenic to both soybean leaves and roots. , , , , , and sp. 1 in FOSC were pathogenic to soybean roots, not to leaves. To our knowledge, this is the first study to thoroughly investigate soybean-associated populations in leaves and roots in Heilongjiang Province.