AI Article Synopsis
- * A new detection method combining photoluminescence and resonance light scattering was developed using CdTe quantum dots and Au nanoparticles, enabling sensitive measurement of miRNA-141 levels.
- * The method achieved a detection limit of 3.7 fM and demonstrated a linear detection range, with dual signals that confirmed accuracy without interference, suggesting a reliable approach for biomarker detection.
Article Abstract
MicroRNAs (miRNAs) hold potential as useful biomarkers for early diagnosis and evaluation of diverse cancers, but their low abundance and short length make the detection of miRNAs face low sensitivity and accuracy. Herein, a photoluminescence (PL)-resonance light scattering (RLS) dual-mode method was developed for the sensitive and accurate detection of miRNA-141 using CdTe quantum dots (QDs) and Au nanoparticles. The presence of miRNA-141 induced PL quenching and RLS increasing. The limit of detection (LOD) was as low as 3.7 fM, and the miRNA-141 was detected linearly in a range from 10 fM to 10 nM. The dual signals generated no mutual interference and were detected using the same spectrophotometer, allowing for mutual validation to ensure the accuracy and reliability of the detection results. This study proposes valuable references for constructing dual-mode detection methods.
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| http://dx.doi.org/10.1016/j.aca.2023.341864 | DOI Listing |
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