AI Article Synopsis
- Primary human nasal epithelial cell (HNEC) cultures are valuable for research on respiratory health, diseases, and treatments.
- The chapter outlines step-by-step protocols for collecting samples and culturing HNECs.
- Culturing is done under air-liquid interface (ALI) conditions to create functional airway cultures with characteristics like beating motile cilia.
Article Abstract
Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the respiratory tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.
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| http://dx.doi.org/10.1007/978-1-0716-3507-0_13 | DOI Listing |
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