Sinomonas cellulolyticus sp. nov., isolated from Loktak lake.

A novel cellulolytic strain JC656 was isolated from the rhizosphere soil of Alisma plantago-aquatica of floating island (Phumdis) of Loktak lake, Manipur, India. The 16S rRNA gene sequence similarities between strain JC656 and other Sinomonas type strains ranged between 98.5 and 97.3%, wherein strain JC656 exhibited the highest sequence similarity (98.5%) to Sinomonas notoginsengisoli KCTC 29237. Colonies were yellow-colored and grew aerobically. Cells were gram-positive, rod-shaped and non-motile. The optimal growth of the strain JC656 occured at 28 °C and pH 7. Strain JC656 contained MK-9 as the predominant isoprenoid quinone and anteiso-C, iso-C and anteiso-C as the major fatty acids. Diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphatidylmonomethylethanolamine and a glycolipid were the polar lipids. Strain JC656 contained lysine, alanine, glutamine, diaminopimelic acid (DAP) and two unidentified amino acids as characteristic cell wall amino acids. The genome size of strain JC656 was 3.9 Mb with a DNA G + C content of 69.9 mol %. For the affirmation of the strain's taxonomic status, a detailed phylogenomic study was done. Based on its phylogenetic position and morphological, physiological, and genomic features, strain JC656 represents a new species of the genus Sinomonas, for which we propose the name Sinomonas cellulolyticus sp. nov. The type strain JC656 = (KCTC 49339 = NBRC 114142).