This study aims to explore the anti-inflammatory mechanisms of sargachromenol in both RAW 264.7 cells and lipopolysaccharide (LPS)-treated mice, as previous reports have suggested that sargachromenol possesses anti-aging, anti-inflammatory, antioxidant, and neuroprotective properties. Although the precise mechanism behind its anti-inflammatory activity remains unclear, pretreatment with sargachromenol effectively reduced the production of nitric oxide, prostaglandin E, and interleukin (IL)-1 in LPS-stimulated RAW 264.7 cells by inhibiting cyclooxygenase-2. Moreover, sargachromenol inhibited the activation of nuclear factor-B (NF-B) by preventing the degradation of the inhibitor of B- (IB-) and inhibiting protein kinase B (Akt) phosphorylation in LPS-stimulated cells. We also found that sargachromenol induced the production of heme oxygenase-1 (HO-1) by activating the nuclear transcription factor erythroid-2-related factor 2 (Nrf2). In LPS-treated mice, oral administration of sargachromenol effectively reduced the levels of IL-1, IL-6, and tumor necrosis factor- (TNF-) in the serum, suggesting its ability to suppress the production of inflammatory mediators by inhibiting the Akt/NF-B pathway and upregulating the Nrf2/HO-1 pathway.
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http://dx.doi.org/10.1055/a-2180-1338 | DOI Listing |
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