LncIRF1 promotes chicken resistance to ALV-J infection.

Unlabelled: The pathogenesis of avian leukosis virus subgroup J (ALV-J) is complex and our understanding of it is limited. Based on our previous research, we explored the relationship between ALV-J infection and regulatory factor 1&7 ( and ), interferon beta (), and the newly identified long noncoding RNA IRF1 (). is 1603 nt and exists in the cytoplasm and nucleus. After the occurrence of ALV-J infection, the expression levels of , , and varied in different chicken tissues. In DF1 cell lines of chicken embryo fibroblast cells (DF1 cells) the expression levels of , , and increased when ALV-J infection. Similarly, after overexpression and the ALV-J challenge, the expression levels of , , and increased, while increased inhibited the proliferation of DF1 cells. Interference with did not affect , , and . However, expression levels of , , and decreased due to interference after the ALV-J challenge. An assay of the RNA-binding domain abundant in apicomplexans indicated that most of the proteins bound to are related to cell proliferation and viral replication and these proteins also interact with IRF1, IRF7, and IFNβ. We suggest that plays an important immunomodulatory role in the anti-ALV-J response.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03773-y.