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Immunomodulatory effects of inactivated Ligilactobacillus salivarius CECT 9609 on respiratory epithelial cells. | LitMetric

AI Article Synopsis

  • The study investigates the immunomodulatory effects of natural products from Ligilactobacillus salivarius CECT 9609 on airway epithelial cells when exposed to the respiratory pathogen Pasteurella multocida.
  • Various forms of the bacteria, including live and inactivated cells, as well as fermentation products, were tested in an established model system.
  • Results showed that only inactivated bacterial cells maintained the structure of respiratory cells while modifying the immune responses against the infection, suggesting potential for further research into their protective benefits.

Article Abstract

The microbiota in humans and animals play crucial roles in defense against pathogens and offer a promising natural source for immunomodulatory products. However, the development of physiologically relevant model systems and protocols for testing such products remains challenging. In this study, we present an experimental condition where various natural products derived from the registered lactic acid bacteria Ligilactobacillus salivarius CECT 9609, known for their immunomodulatory activity, were tested. These products included live and inactivated bacteria, as well as fermentation products at different concentrations and culture times. Using our established model system, we observed no morphological changes in the airway epithelium upon exposure to Pasteurella multocida, a common respiratory pathogen. However, early molecular changes associated with the innate immune response were detected through transcript analysis. By employing diverse methodologies ranging from microscopy to next-generation sequencing (NGS), we characterized the interaction of these natural products with the airway epithelium and their potential beneficial effects in the presence of P. multocida infection. In particular, our discovery highlights that among all Ligilactobacillus salivarius CECT 9609 products tested, only inactivated cells preserve the conformation and morphology of respiratory epithelial cells, while also reversing or altering the natural immune responses triggered by Pasteurella multocida. These findings lay the groundwork for further exploration into the protective role of these bacteria and their derivatives.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580541PMC
http://dx.doi.org/10.1186/s13567-023-01228-zDOI Listing

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