Investigating synergism and mechanism during sequential inactivation of Staphylococcus aureus with ultrasound followed by UV/peracetic acid.

This study explored the inactivation of Staphylococcus aureus (S. aureus) by ultrasound (US) and peracetic acid (PAA) coupling with UV simultaneously (US/PAA/UV) or sequentially (US→PAA/UV) for the strengthened disinfection. The result showed that US→PAA/UV system had excellent inactivation performance with 5.05-log in a short time. Besides US, UV, PAA and free radicals, the contribution of the synergy of all components to the entire disinfection were obvious under US→PAA/UV system. The inactivation performance of S. aureus significantly decreased with the increase of humic acid (HA) concentration and pH; however, the rising temperature contributes to the enhancement of the inactivation efficiency under the US→PAA/UV system. The disinfection mechanism includes a decrease of cell agglomeration, a loss of intracellular substance, and changes of cell structure and membrane permeability, as evidenced through a nanoparticle size analyzer, scanning electron microscope (SEM), transmission electron microscope (TEM) and laser confocal microscopy (LSCM). Furthermore, the inactivation efficiency of the US→PAA/UV system for the total bacteria from actual sewage (the untreated inflow) was high, which reached 3.86-log. In general, the pretreatment of US combined with UV/PAA showed a promising application in the rapid disinfection of sewage.