Metabolic pathway of tebuconazole by soil fungus Cunninghamella elegans ATCC36112.

Antonie Van Leeuwenhoek

College of Chemistry and Pharmacy, Qingdao Agricultural University, Changcheng Rd, Chengyang, Qingdao City, 266-109, Shandong Province, China.

Published: December 2023

AI Article Synopsis

  • * A study on the metabolism of tebuconazole in Cunninghamella elegans showed that about 98% of it was degraded within 7 days, resulting in five identified metabolites.
  • * The study identified cytochrome P450 and flavin-dependent monooxygenase as key enzymes in the metabolic process, providing insights for understanding the toxicological effects and potential bioremediation of tebuconazole.

Article Abstract

Tebuconazole is the most widely used fungicide in agriculture. Due to its long half-life, tebuconazole residues can be found in the environment media such as in soil and water bodies. Here, the metabolic pathway of tebuconazole was studied in Cunninghamella elegans (C. elegans). Approximately 98% of tebuconazole was degraded within 7 days, accompanied by the accumulation of five metabolites. The structures of the metabolites were completely or tentatively identified by gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). To identify representative oxidative enzymes that may be involved in the metabolic process, treatment with piperonyl butoxide (PB) and methimazole (MZ) was performed. PB had a strong inhibitory effect on the metabolic reactions, while MZ had a weak inhibitory effect. The results suggest that cytochrome P450 (CYP) and flavin-dependent monooxygenase are involved in the metabolism of tebuconazole. Based on the results, we propose a metabolic pathway for the fungal metabolism of tebuconazole. Data are of interest to gain insight into the toxicological effects of tebuconazole and for tebuconazole bioremediation.

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Source
http://dx.doi.org/10.1007/s10482-023-01894-1DOI Listing

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