Prevalence and epidemiological characteristics of methicillin-resistant isolated from cattle in Bangalore India as a part of the One Health approach.

In India, limited studies are available on the epidemiological aspects of methicillin-resistant (MRSA) infections in both animal and human settings. Herein, we investigated the prevalence, antimicrobial resistance profile and molecular characteristics of MRSA isolates recovered from cattle using the One Health approach. Out of 66 A-positive staphylococci, species-specific multiplex PCR detected 24 % (=16) of isolates as MRSA. Maximum antibiotic resistance was seen against cloxacillin (94 %, =15) and least for enrofloxacin and cephalothin (each 13 %, =2). Overall, 13 % (=2) of MRSA isolates were multidrug-resistant. Molecular characterization by SCC typing identified 88 % (=14) of MRSA isolates as type V. Twelve isolates (75 %) belonged to novel -type t17242, of which 67 % (=8) belonged to type I. MLST analysis revealed ST 1687 (50 %, =8) as the most predominant sequence type. Circulation of different MRSA clones among the cattle populace offers a risk of transmission to humans through direct contact, food chain or environmental contamination. Thus, continuous monitoring of MRSA strains is imperative for early diagnosis and for establishing effective treatment strategies to restrain the disease burden caused by MRSA infections.