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Regulation of dermal fibroblasts by human neutrophil peptides. | LitMetric

Regulation of dermal fibroblasts by human neutrophil peptides.

Sci Rep

Center of Excellence in Translational Research in Inflammation and Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Published: October 2023

AI Article Synopsis

  • The study explores how human neutrophil peptides (HNPs) affect human dermal fibroblasts, showing they can promote cell growth and activation without toxicity.
  • HNP1 treatment significantly increased the expression of genes related to cell proliferation (Ki-67) and activation (COL1A1) in both 2D and 3D cultures, enhancing collagen production.
  • RNA sequencing revealed distinct gene expression patterns between HNP1-treated and control cells, indicating that HNPs boost cell activity while downregulating genes involved in lipid metabolism and inflammation.

Article Abstract

Human neutrophil peptides (HNPs) can induce cell proliferation and activation so their growth promoting activities may have potential clinical benefit. This study investigated the effects of HNPs on human dermal fibroblasts. Differential gene expression in HNP-treated cells and genes involved in regulating intracellular pathways were explored. Dermal fibroblasts were isolated from healthy neonatal foreskin and treated with HNPs in 2D and 3D cell culture systems. The expression of cell proliferation (Ki-67) gene and cell activation (COL1A1) gene plus their proteins was measured. Differential gene expression was determined using RNA-seq, and upregulated and downregulated genes were mapped onto intracellular pathways by KEGG analysis and Gene Ontology databases. HNPs significantly increased cell proliferation without cytotoxicity whilst HNP1 enhanced expression of COL1A1 and type I collagen production in 2D cells and 3D spheroids. RNA-sequencing analysis showed gene clustering with clear separation between HNP1-treated and control groups. A heatmap of top 50 differentially expressed genes was consistent among HNP1-treated samples. Most upregulated genes were associated with cell proliferation and activation as mapped into intracellular pathways whilst most downregulated genes belonged to steroid/arachidonic acid metabolism and inflammatory signaling pathways. HNP1 increased cell proliferation and activation but reduced lipid metabolism and inflammation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577140PMC
http://dx.doi.org/10.1038/s41598-023-44889-8DOI Listing

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