AI Article Synopsis

  • The project aimed to create probiotic powders for lung administration to help control pathogen growth in patients with lung infections.
  • The spray drying method used successfully preserved the viability of the probiotics, with only a slight decrease over 30 days.
  • Findings indicated that the powders are respirable and safe for lung use, with one probiotic strain showing both bactericidal and bacteriostatic effects, suggesting potential benefits in cystic fibrosis treatment and microbiota restoration.

Article Abstract

Objectives: The aim of the project was to develop and characterise powders containing a probiotic (Lactiplantibacillus plantarum [Lpb. plantarum], Lacticaseibacillus rhamnosus, or Lactobacillus acidophilus) to be administered to the lung for the containment of pathogen growth in patients with lung infections.

Methods: The optimised spray drying process for the powder manufacturing was able to preserve viability of the bacteria, which decreased of only one log unit and was maintained up to 30 days.

Results: Probiotic powders showed a high respirability (42%-50% of particles had a size < 5 µm) suitable for lung deposition and were proven safe on A549 and Calu-3 cells up to a concentration of 10 colony-forming units/mL. The Lpb. plantarum adhesion to both cell lines tested was at least 10%. Surprisingly, Lpb. plantarum powder was bactericidal at a concentration of 10 colony-forming units/mL on P. aeruginosa, whereas the other two strains were bacteriostatic.

Conclusion: This work represents a promising starting point to consider a probiotic inhalation powder a value in keeping the growth of pathogenic microflora in check during the antibiotic inhalation therapy suspension in cystic fibrosis treatment regimen. This approach could also be advantageous for interfering competitively with pathogenic bacteria and promoting the restoration of the healthy microbiota.

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Source
http://dx.doi.org/10.1016/j.ijantimicag.2023.107001DOI Listing

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