Three Strains of Derived from Piglets Alleviated Intestinal Oxidative Stress Induced by Diquat through Extracellular Vesicles.

Previous studies found that polysaccharides (PCPs) significantly enhanced the antioxidant activity in piglet intestines while increasing the abundance of . However, the relationship between and antioxidant activity has yet to be verified, and the mode of action needs further investigation. Six strains isolated from the intestines of neonatal piglets fed with PCPs were studied to investigate the relationship between and intestinal oxidative stress. The results showed that three of them alleviated intestinal oxidative stress and protected the intestinal barrier. Subsequently, we extracted the extracellular vesicles (EVs) of these three strains to verify their intestinal protection mode of action. We found that these EVs exerted an excellent antioxidant effect and intestinal barrier protection and could directly improve intestinal microbial composition. Our findings suggested that the EVs of the three strains could enhance antioxidant activity by improving the physical intestinal barrier and remodeling gut microbiota. Unlike probiotics, which should be pre-colonized, EVs can act directly on the intestines. This study provides new ideas for the subsequent development of products to protect intestinal health.