Two Gram-stain-negative, catalase- and oxidase-positive, aerobic non-motile and motile rod bacteria, strains MSW6 and RSW2, were isolated from surface seawater. Strain MSW6 optimally grew at 20 °C, pH 7.0 and 3 % NaCl, while strain RSW2 optimally grew at 25 °C, pH 7.0-8.0 and 2 % NaCl. Strain MSW6 possessed menaquinone-6 as the major respiratory quinone, and its major fatty acids were iso-C G, iso-C and iso-C 3-OH. The major polar lipid identified in strain MSW6 was phosphatidylethanolamine (PE). On the other hand, strain RSW2 had ubiquinone-8 as the predominant respiratory quinone, and its major fatty acids consisted of summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6) and C. The major polar lipids identified in strain RSW2 were PE and phosphatidylglycerol. As the sole respiratory quinone, strain MSW6 possessed menaquinone-6, while strain RSW2 had ubiquinone-8. The DNA G+C contents of strains MSW6 and RSW2 were 31.9 and 43.4 mol%, respectively. Phylogenetic analyses based on 16S rRNA and core gene sequences showed that strain MSW6 formed a phylogenic lineage with KOPRI 13649, while strain RSW2 formed a phylogenic lineage with KMM 3633. Strain MSW6 shared 97.9 % 16S rRNA gene sequence similarity and 80.7 % average nucleotide identity (ANI) ith KOPRI 13649, and strain RSW2 shared 99.1 % 16S rRNA gene sequence similarity and 93.1 % ANI with KMM 3633. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strains MSW6 and RSW2 represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of is MSW6 (=KACC 22338=JCM 35022) and the type strain of is RSW2 (=KACC 22716=JCM 35550).
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http://dx.doi.org/10.1099/ijsem.0.006090 | DOI Listing |
Int J Syst Evol Microbiol
October 2023
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Mycobiology
February 2020
Environmental Microbiology Lab, Department of Agricultural Biological Chemistry, College of Agriculture & Life Sciences, Chonnam National University, Gwangju, Korea.
During an investigation of the fungi from the Aspergillaceae family obtained from different environmental sources in Korea, we isolated six strains, including CNUFC WJC9-1, CNUFC BPM36-33, CNUFC MSW6, CNUFC ESW1, CNUFC TM6-2, and CNUFC WD17-1. The morphology and phylogeny of these isolates were analyzed based on their partial -tubulin () and calmodulin () gene sequences. Based on the morphological characteristics and sequence analyses, the isolates CNUFC WJC9-1, CNUFC BPM36-33, CNUFC TM6-2, and CNUFC WD17-1 were identified as , , , and , respectively, and isolates CNUFC MSW6 and CNUFC ESW1 were identified as .
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