sp. nov. and sp. nov., isolated from seawater.

Int J Syst Evol Microbiol

Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.

Published: October 2023

AI Article Synopsis

  • Two new rod-shaped bacteria strains, MSW6 and RSW2, were isolated from seawater; they differ in optimal growth conditions and respiratory quinone types.
  • Strain MSW6 thrives at 20°C with 3% NaCl and uses menaquinone-6, while strain RSW2 prefers 25°C with 2% NaCl and has ubiquinone-8.
  • Phylogenetic analysis indicates strain MSW6 is closely related to KOPRI 13649 with significant gene similarity, whereas strain RSW2 is closely related to KMM 3633, leading to the proposal of them as novel species.

Article Abstract

Two Gram-stain-negative, catalase- and oxidase-positive, aerobic non-motile and motile rod bacteria, strains MSW6 and RSW2, were isolated from surface seawater. Strain MSW6 optimally grew at 20 °C, pH 7.0 and 3 % NaCl, while strain RSW2 optimally grew at 25 °C, pH 7.0-8.0 and 2 % NaCl. Strain MSW6 possessed menaquinone-6 as the major respiratory quinone, and its major fatty acids were iso-C G, iso-C and iso-C 3-OH. The major polar lipid identified in strain MSW6 was phosphatidylethanolamine (PE). On the other hand, strain RSW2 had ubiquinone-8 as the predominant respiratory quinone, and its major fatty acids consisted of summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6) and C. The major polar lipids identified in strain RSW2 were PE and phosphatidylglycerol. As the sole respiratory quinone, strain MSW6 possessed menaquinone-6, while strain RSW2 had ubiquinone-8. The DNA G+C contents of strains MSW6 and RSW2 were 31.9 and 43.4 mol%, respectively. Phylogenetic analyses based on 16S rRNA and core gene sequences showed that strain MSW6 formed a phylogenic lineage with KOPRI 13649, while strain RSW2 formed a phylogenic lineage with KMM 3633. Strain MSW6 shared 97.9 % 16S rRNA gene sequence similarity and 80.7 % average nucleotide identity (ANI) ith KOPRI 13649, and strain RSW2 shared 99.1 % 16S rRNA gene sequence similarity and 93.1 % ANI with KMM 3633. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strains MSW6 and RSW2 represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of is MSW6 (=KACC 22338=JCM 35022) and the type strain of is RSW2 (=KACC 22716=JCM 35550).

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http://dx.doi.org/10.1099/ijsem.0.006090DOI Listing

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sp. nov. and sp. nov., isolated from seawater.

Int J Syst Evol Microbiol

October 2023

Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.

Article Synopsis
  • Two new rod-shaped bacteria strains, MSW6 and RSW2, were isolated from seawater; they differ in optimal growth conditions and respiratory quinone types.
  • Strain MSW6 thrives at 20°C with 3% NaCl and uses menaquinone-6, while strain RSW2 prefers 25°C with 2% NaCl and has ubiquinone-8.
  • Phylogenetic analysis indicates strain MSW6 is closely related to KOPRI 13649 with significant gene similarity, whereas strain RSW2 is closely related to KMM 3633, leading to the proposal of them as novel species.
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