A multiscale study of the effects of a diet containing CdSe/ZnS-COOH quantum dots on Salmo trutta fario L.: Potential feed-related nanotoxicity.

Quantum dots (QDs) receive widespread attention in industrial and biomedical fields, but the risks posed by the use of nanoparticles to aquatic organisms and the associated toxicological effects are still not well understood. In this study, effects of the 7-day dietary exposure of Salmo trutta fario L. juveniles to CdSe/ZnS-COOH QDs were evaluated at molecular, cellular, physiological and whole-organism levels. Fish feeding with QDs-contaminated feed resulted in an increased somatic index of the liver, a genotoxic effect on peripheral blood erythrocytes, altered enzyme activity and decreased MDA level. Furthermore, Cd levels in the gills and liver tissues of the exposed fish were found to be significantly higher than in those of the control fish. Alpha diversity indexes of the gut microbiota of the QDs-exposed S. trutta fario L. individuals exhibited a decreasing trend. The principal coordinate analysis (PCoA) showed that the gut microbiota of the control fish was significantly different from that of the fish exposed to QDs (p < 0.05). Additionally, the linear discriminant analysis (LDA) performed using an effect size (LEfSe) algorithm unveiled 19 significant taxonomic differences at different taxonomic levels between the control group and the QDs-exposed group. In the QDs-exposed group, the relative abundance of the genus Citrobacter (Proteobacteria phylum) in the gut microbiota was found to be significantly increased whereas that of the genus Mycoplasma (Tenericutes phylum) significantly decreased compared to the control group. In summary, QDs-contaminated diet affects the gut microbiota of fish by significantly changing the relative abundance of some taxa, potentially leading to dysbiosis. This, together with morphophysiological, cytogenetic and biochemical changes, poses a risk to fish health.