"Lysis and Concentration Technique" improves the parasitological diagnosis of Trypanosoma vivax.

Vet Parasitol

São Paulo State University (Unesp), School of Agricultural and Veterinary Sciences, Veterinary Medicine Post-Graduation Program Jaboticabal, Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, SP, Brazil; São Paulo State University (Unesp), School of Veterinary Medicine, Araçatuba, Rua Clóvis Pestana 793, 16050-470 Araçatuba, SP, Brazil. Electronic address:

Published: November 2023

The "Lysis and Concentration Technique" (LCTe) involves lysing red blood cells and concentrating parasites to increase the chances of visualization in low parasitemia and in scenarios of evaluators with less knowledge. The lysis of red blood cells reduced the time of diagnosis by 21 s, showing that the effect produced by the treatment is comparable to the effect of the experience in parasitological examination. In addition, the concentration of parasites was 39.18% higher for slides with high parasitemia and 131.03% for slides with low parasitemia in relation to the standard slide. LCTe proved to be inexpensive, with a total cost of approximately US$0.07 per slide made, which allows it to be easily implemented in most laboratories.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetpar.2023.110043DOI Listing

Publication Analysis

Top Keywords

"lysis concentration
8
concentration technique"
8
red blood
8
blood cells
8
low parasitemia
8
technique" improves
4
improves parasitological
4
parasitological diagnosis
4
diagnosis trypanosoma
4
trypanosoma vivax
4

Similar Publications

Polyamidoamine (PAMAM) dendrimers, with their unique structural versatility and tunable surface functionalities, have emerged as promising nanomaterials for a wide range of biomedical applications. However, their in vivo use raises concerns, as unintended interactions between dendrimers and blood components could disrupt the delicate hemostatic balance and lead to serious complications like bleeding or thrombosis. In this study, we explored the impact of low-generation PAMAM dendrimers on the kinetics of fibrin clot formation, along with their influence on the structure, properties, and resistance to lysis of the resulting clots.

View Article and Find Full Text PDF

Antibiotic resistance genes (ARGs) have been emerging as a concerning threat to both environment and public health. The continuous input of manure, irrigation water, and fertilizers increases the abundance of ARGs in agricultural environments. However, current risk assessments have focused on clinical settings, which are not applicable to environmental settings.

View Article and Find Full Text PDF

Tuberculosis (TB) is the second deadliest infectious disease worldwide. Current TB diagnostics utilize sputum samples, which are difficult to obtain, and sample processing is time-consuming and difficult. This study developed an integrated diagnostic platform for the rapid visual detection of Mycobacterium tuberculosis (Mtb) in breath samples at the point-of-care (POC), especially in resource-limited settings.

View Article and Find Full Text PDF
Article Synopsis
  • Hard tick exoskeletons make DNA extraction difficult, prompting researchers to test a modified method for extracting DNA from ethanol-preserved ticks for genetic studies.
  • The new method was compared to three commercial kits and showed similar DNA concentration and purity across different life stages of ticks.
  • The extracted DNA was used for PCR amplification of phylogenetic markers to analyze Amblyomma integrum, a potential disease vector, demonstrating a cost-effective approach that can aid genetic research in low-resource settings.
View Article and Find Full Text PDF

Potential of Bacillus thuringiensis isolates to manage Gonipterus platensis (Coleoptera: Curculionidae) larvae populations.

Environ Entomol

December 2024

Departamento de Proteção Vegetal, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP), Faculdade de Ciências Agronômicas, Avenida Universitária, Botucatu, São Paulo, Brasil.

The growing expansion of eucalyptus plantations in Brazil and the impact of exotic pests, such as Gonipterus platensis, demand effective, and sustainable biological control strategies. The aim of this study was to assess the pathogenicity of 10 Bacillus thuringiensis (Bt) isolates to neonate Gonipterus platensis larvae, commonly known as the eucalyptus weevil (Coleoptera: Curculionidae) with the specific focus of evaluating their potential to manage this pest while preserving its egg parasitoid, Anaphes nitens. To achieve this, the genomic DNA of the 10 Bt isolates was extracted using the thermal lysis method for molecular characterization of their Cry and Vip proteins.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!