AI Article Synopsis
- Single-cell transcriptomic studies have discovered a consistent set of neocortical cell types from small postmortem samples, and this research expanded on that by analyzing cell type variation in 75 adult patients undergoing brain surgeries.
- The study found that nearly all identified nuclei fit into one of 125 established cell types from the middle temporal gyrus, but revealed significant variations among individuals, especially in deep-layer glutamatergic neurons and microglia.
- Factors like age, sex, ancestry, and disease state explained only a small portion of this variance, indicating that genomic differences were linked to the expression of 150 to 250 genes for most cell types, providing a reference for understanding cellular variations in health and disease.
Article Abstract
Single-cell transcriptomic studies have identified a conserved set of neocortical cell types from small postmortem cohorts. We extended these efforts by assessing cell type variation across 75 adult individuals undergoing epilepsy and tumor surgeries. Nearly all nuclei map to one of 125 robust cell types identified in the middle temporal gyrus. However, we found interindividual variance in abundances and gene expression signatures, particularly in deep-layer glutamatergic neurons and microglia. A minority of donor variance is explainable by age, sex, ancestry, disease state, and cell state. Genomic variation was associated with expression of 150 to 250 genes for most cell types. This characterization of cellular variation provides a baseline for cell typing in health and disease.
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| http://dx.doi.org/10.1126/science.adf2359 | DOI Listing |
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