https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=37824076&retmode=xml&tool=Litmetric&email=readroberts32@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09 378240762023110120231107
1940-602927232024Methods in molecular biology (Clifton, N.J.)Methods Mol BiolDifferential Poly(A) Tail Length Analysis Using Nanopore Sequencing.267283267-28310.1007/978-1-0716-3481-3_16The poly(A) tail is a sequence of several adenosine nucleotides added to the 3' end of RNA molecules transcribed by polymerase II. The dynamics of poly(A) tail length play a significant role in regulating post-transcriptional gene expression by regulating the stability, translation, and decay of messenger RNAs. As a result, an accurate measurement of poly(A) tail length changes is important for understanding its regulatory function in different cellular contexts. Here, we outline a method for using nanopore sequencing and linear mixed models to analyze differences in poly(A) tail length across conditions.© 2024. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.DarShowkat ASALaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.MallaSulochanSLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.BelairCedricCLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.MaragkakisManolisMLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA. emmanouil.maragkakis@nih.gov.engZIA AG000696ImNIHIntramural NIH HHSUnited StatesJournal ArticleResearch Support, N.I.H., Intramural
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