AI Article Synopsis
- - The study focuses on the role of calcium in bone health and examines how isoflavones (like daidzein and genistein) and probiotics affect calcium transporters in healthy female rats, aiming to understand their impact on bone metabolism and serum calcium levels.
- - Results indicated that a diet rich in daidzein and genistein significantly increased gene expression of the Trpv6 calcium transporter in the duodenum and lowered serum levels of pyridinoline, a bone metabolism biomarker, but no major effects were seen with tempeh and soybean diets.
- - Overall, the findings suggest that while isoflavones may enhance calcium transport and reduce certain bone biomarkers, the combination of these dietary components did not result
Article Abstract
Calcium is essential for maintaining bone health as it contributes to bone formation, remodeling, strength, and density. This study investigated the effect of isoflavones and probiotics on calcium transporters' gene expression, serum calcium levels, and bone metabolism biomarkers in healthy female rats. Forty-eight female Wistar rats were classified into six groups. Bone metabolism biomarkers (pyridinoline, deoxypyridinoline, parathyroid hormone, and osteocalcin) and serum calcium levels were measured by enzyme-linked immunosorbent assay (ELISA) and atomic absorption spectroscopy (AAS), respectively. Gene expression of calcium transporters (Trpv5 and Trpv6) was evaluated in duodenum and jejunum tissue samples using quantitative polymerase chain reaction (qPCR). Trpv5 and Trpv6, epithelial calcium channels, play a crucial role in calcium transport and homeostasis in the body. The study consisted of a1-week adaptation period for the rats to adjust to the controlled conditions, followed by an 8-week intervention phase. The daidzein and genistein group showed a significant increase in the gene expression of the Trpv6 transporter in the duodenum and a marked decrease in serum pyridinoline levels compared to the control group. The tempeh and soybean groups showed a significant decrease in the gene expression of the Trpv5 calcium transporter in the jejunum. However, no significant influence of the diet on calcium transport and bone metabolism biomarkers was observed in the group. The correlation analysis showed a significant positive relationship between serum calcium, bone metabolism biomarkers, and calcium transporters. In conclusion, our study demonstrates that the daidzein and genistein diet improves calcium transport in the duodenum and reduces pyridinoline serum concentrations, while tempeh and soybean diets reduce calcium transport in the jejunum. However, the combination of daidzein, genistein, and did not demonstrate a synergistic effect on calcium transport and bone metabolism, suggesting that further investigations are needed to elucidate their potential interactions.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563734 | PMC |
| http://dx.doi.org/10.1002/fsn3.3571 | DOI Listing |
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