AI Article Synopsis
- Naive T lymphocytes move through the body to find antigens, switching between blood and lymph nodes, with their movement influenced by specific chemokines.
- The study investigates how T lymphocytes respond to the chemokine CCL21 and sphingolipid S1P using advanced techniques to observe real-time behavior.
- Results indicate that CCL21 leads to strong and sustained movement toward lymph nodes, while S1P only causes temporary polarization, suggesting that S1P is more about quick exit rather than long-distance movement.
Article Abstract
Naive T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naive T lymphocyte migration along defined gradients is missing. Here, we used a reductionist approach to study the real-time single-cell response of naive T lymphocytes to CCL21 and serum rich in bioactive S1P. Using microfluidic and micropatterning ad hoc tools, we show that CCL21 triggers stable polarization and long-range chemotaxis of cells, whereas S1P-rich serum triggers a transient polarization only and no significant displacement, potentially representing a brief transmigration step through exit portals. Our data thus suggest that naive T lymphocyte chemotax long distances to CCL21 but not toward a source of bioactive S1P.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562802 | PMC |
| http://dx.doi.org/10.1016/j.isci.2023.107695 | DOI Listing |
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