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Engineering dense tumor constructs via cellular contraction of extracellular matrix hydrogels. | LitMetric

AI Article Synopsis

  • The study explores how the physical characteristics of solid tumors, like stiffness and internal structure, affect cancer growth and treatment.
  • Researchers developed a method to create tissue-engineered constructs that mimic dense solid tumors by allowing human fibroblasts to contract and compact collagen hydrogels filled with cancer cells.
  • This novel approach promises to improve models for studying cancer behavior and could enhance the delivery of treatments such as drug therapies and immunotherapies.

Article Abstract

Physical characteristics of solid tumors such as dense internal microarchitectures and pathological stiffness influence cancer progression and treatment. While it is routine to engineer culture substrates and scaffolds with elastic moduli that approximate tumors, these models often fail to capture characteristic internal microarchitectures such as densely compacted concentric ECM fibers at the stromal interface. Contractile mesenchymal cells can solve this engineering challenge by deforming, contracting, and compacting extracellular matrix (ECM) hydrogels to decrease tissue volume and increase tissue density. Here we demonstrate that allowing human fibroblasts of varying origins to freely contract collagen type I-containing hydrogels co-seeded with carcinoma cell spheroids produces a tissue engineered construct with structural features that mimic dense solid tumors in vivo. Morphometry and mechanical testing were conducted in tandem with biochemical analysis of proliferation and viability to confirm that dense carcinoma constructs engineered using this approach capture relevant physical characteristics of solid carcinomas in a tractable format that preserves viability and is amenable to extended culture. The reported method is adaptable to the use of multiple mesenchymal cell types and the inclusion of fibrin in the ECM combined with seeding of endothelial cells to produce prevascularized constructs. The physical dense carcinoma constructs engineered using this approach may provide more clinically relevant venues for studying cancer pathophysiology and the challenges associated with the delivery of macromolecular drugs and cellular immunotherapies to solid tumors.

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Source
http://dx.doi.org/10.1002/bit.28561DOI Listing

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