AI Article Synopsis
- Electrochemiluminescence (ECL) is emerging as a powerful microscopy technique due to its optical readout and ability to visualize various microscopic entities.
- The study focuses on the dynamic imaging of single [Ru(bpy)]-functionalized beads and reveals the reaction kinetics of ECL using tripropylamine as a co-reactant.
- This dynamic imaging approach enables a deeper understanding of ECL emission behavior, layer thickness variations, and intensity changes over time, offering insights into the kinetics and mechanisms relevant to bioassays and cell microscopy that static imaging could not provide.
Article Abstract
As an electrochemical technique offering an optical readout, electrochemiluminescence (ECL) evolved recently into a powerful microscopy technique with the visualization of a wide range of microscopic entities. However, the dynamic imaging of transient ECL events did not receive intensive attention due to the limited number of electrogenerated photons. Here, the reaction kinetics of the model ECL bioassay system was revealed by dynamic imaging of single [Ru(bpy)]-functionalized beads in the presence of the efficient tripropylamine coreactant. The time profile behavior of ECL emission, the variations of the ECL layer thickness, and the position of maximum ECL intensity over time were investigated, which were not achieved by static imaging in previous studies. Moreover, the dynamics of the ECL emission were confronted with the simulation. The reported dynamic ECL imaging allows the investigation of the ECL kinetics and mechanisms operating in bioassays and cell microscopy.
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| http://dx.doi.org/10.1021/acs.analchem.3c02960 | DOI Listing |
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