AI Article Synopsis
- Mapping the synaptic connections in a mammalian brain can significantly enhance our understanding of perception, behavior, and memory.
- The traditional method of using serial section electron microscopy faces challenges with chemical treatment and tissue volume changes, which can compromise the quality of the samples.
- A new 12-step protocol called ODeCO allows for effective osmium infiltration in mouse brains, preserving ultrastructure and preventing cracks, helping in the creation of a complete mouse brain connectome.
Article Abstract
Mapping the complete synaptic connectivity of a mammalian brain would be transformative, revealing the pathways underlying perception, behavior, and memory. Serial section electron microscopy, via membrane staining using osmium tetroxide, is ideal for visualizing cells and synaptic connections but, in whole brain samples, faces significant challenges related to chemical treatment and volume changes. These issues can adversely affect both the ultrastructural quality and macroscopic tissue integrity. By leveraging time-lapse X-ray imaging and brain proxies, we have developed a 12-step protocol, ODeCO, that effectively infiltrates osmium throughout an entire mouse brain while preserving ultrastructure without any cracks or fragmentation, a necessary prerequisite for constructing the first comprehensive mouse brain connectome.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557665 | PMC |
| http://dx.doi.org/10.1101/2023.09.26.558265 | DOI Listing |
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