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RNA Translocation through Protein Nanopores: Interlude of the Molten RNA Globule.
J Am Chem Soc
January 2025
Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts 01003, United States.
Direct translocation of RNA with secondary structures using single-molecule electrophoresis through protein nanopores shows significant fluctuations in the measured ionic current, in contrast to unstructured single-stranded RNA or DNA. We developed a multiscale model combining the oxRNA model for RNA with the 3-dimensional Poisson-Nernst-Planck formalism for electric fields within protein pores, aiming to map RNA conformations to ionic currents as RNA translocates through three protein nanopores: α-hemolysin, CsgG, and MspA. Our findings reveal three distinct stages of translocation (pseudoknot, melting, and molten globule) based on contact maps and current values.
View Article and Find Full Text PDFEffect of Cawthorne and Cooksey exercise on balance control and fall risk among middle-aged population: A single group pre-post experimental study.
J Educ Health Promot
November 2024
Institute of Physiotherapy, Srinivas University, City Campus, Pandeshwar, Mangalore, Karnataka, India.
Background: Balance impairment is one of the causes of the risk of falls among all age groups and is a continuing threat to morbidity and mortality across the world. Previous studies stated that the vestibular system is affected in middle-aged population, which leads to imbalance and a high risk of falls. There is a lack of research in this account so this study has been done to find the effect of Cawthorne and Cooksey exercise in vestibular rehabilitation on balance control and the risk of falls among middle-age population.
View Article and Find Full Text PDFDetermination of spin Hall effect and spin diffusion length of Pt from self-consistent fitting of damping enhancement and inverse spin-orbit torque measurements.
Phys Rev B
January 2018
Quantum Electromagnetics Division, National Institute of Standards and Technology, Boulder, CO 80305, U.S.A.
Understanding the evolution of spin-orbit torque (SOT) with increasing heavy-metal thickness in ferromagnet/normal metal (FM/NM) bilayers is critical for the development of magnetic memory based on SOT. However, several experiments have revealed an apparent discrepancy between damping enhancement and damping-like SOT regarding their dependence on NM thickness. Here, using linewidth and phase-resolved amplitude analysis of vector network analyzer ferromagnetic resonance (VNA-FMR) measurements, we simultaneously extract damping enhancement and both field-like and damping-like inverse SOT in NiFe/Pt bilayers as a function of Pt thickness.
View Article and Find Full Text PDFGlobal regulators enable bacterial adaptation to a phenotypic trade-off.
iScience
January 2025
Laboratoire de Biochimie, UMR CNRS-ESPCI 8231 Chimie Biologie Innovation, PSL Research University, ESPCI Paris, 10 Rue Vauquelin, 75005 Paris, France.
Cellular fitness depends on multiple phenotypes that must be balanced during evolutionary adaptation. For instance, coordinating growth and motility is critical for microbial colonization and cancer invasiveness. In bacteria, these phenotypes are controlled by local regulators that target single operons, as well as by global regulators that impact hundreds of genes.
View Article and Find Full Text PDFCenter of mass acceleration during walking: comparison between IMU and camera-based motion capture methodologies.
Wearable Technol
November 2024
Department of Kinesiology, Iowa State University, Ames, IA, USA.
Placing an inertial measurement unit (IMU) at the 5th lumbar vertebra (L5) is a frequently employed method to assess the whole-body center of mass (CoM) motion during walking. However, such a fixed position approach does not account for instantaneous changes in body segment positions that change the CoM. Therefore, this study aimed to assess the congruence between CoM accelerations obtained from these two methods.
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