AI Article Synopsis
- Poria is a significant medicinal herb, and researchers have identified a polysaccharide called PCP-Ⅰ from it, which enhances the effectiveness of vaccines better than conventional aluminum adjuvants.
- The study used various analytical techniques, including hydrolysis and spectroscopy, to explore the chemical structure of PCP-Ⅰ, revealing it primarily consists of galactose with specific linkages.
- Understanding the structure of PCP-Ⅰ may help in developing improved vaccine adjuvants for better immunological responses.
Article Abstract
Poria is an important medical herb in clinic. The authors isolated a polysaccharide(PCP-Ⅰ) from Poria in previous studies, which is composed of galactose, mannose, fucose and glucose. PCP-Ⅰ exhibited significant adjuvant effects on H1N1 influenza vaccine, hepatitis B surface antigen and anthrax protective antigen, and its adjuvant activity was stronger than aluminium adjuvant. However, little is known about the chemical structure of PCP-Ⅰ at present. In this study, weak acid hydrolysis was used to obtain the backbone oligosaccharide of PCP-Ⅰ. Then periodate oxidation, Smith degradation, methylation analysis, Fourier transform infrared spectroscopy(FT-IR), nuclear magnetic resonance(NMR) and gas chromatography-mass spectrometry(GC-MS) were performed to investigate the chemical structural features of PCP-Ⅰ and its hydrolytic oligosaccharide(PCP-Ⅰ-hy-1). These results suggested that the backbone of PCP-Ⅰ was composed of galactose with α anomeric carbon and β anomeric carbon. The linking residues of galactan are(1→),(l→6) and(1→2,6).
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| http://dx.doi.org/10.19540/j.cnki.cjcmm.20230124.201 | DOI Listing |
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