Ectoines production from biogas in pilot bubble column bioreactors and their subsequent extraction via bio-milking.

Water Res

Institute of Sustainable Processes, University of Valladolid, Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n., Valladolid 47011, Spain. Electronic address:

Published: October 2023

AI Article Synopsis

  • Biogas has renewable energy potential but faces challenges due to pollutants and competition from cheaper solar and wind energy sources.
  • Research focused on a 20 L bubble column bioreactor and two interconnected bioreactors for producing ectoine from biogas, finding that the stand-alone reactor performed best in both efficiency and robustness for ectoine accumulation and methane degradation.
  • A method called bacterial bio-milking effectively recovered ectoine with over 70% efficiency, while recycling methanotrophic cells did not negatively impact methane degradation or ectoine production.

Article Abstract

Despite the potential of biogas from waste/wastewater treatment as a renewable energy source, the presence of pollutants and the rapid decrease in the levelized cost of solar and wind power constrain the use of biogas for energy generation. Biogas conversion into ectoine, one of the most valuable bioproducts (1000 €/kg), constitutes a new strategy to promote a competitive biogas market. The potential for a stand-alone 20 L bubble column bioreactor operating at 6% NaCl and two 10 L interconnected bioreactors (at 0 and 6% NaCl, respectively) for ectoine production from biogas was comparatively assessed. The stand-alone reactor supported the best process performance due to its highest robustness and efficiency for ectoine accumulation (20-52 mg/g) and CH degradation (up to 84%). The increase in N availability and internal gas recirculation did not enhance ectoine synthesis. However, a 2-fold increase in the internal gas recirculation resulted in an approximately 1.3-fold increase in CH removal efficiency. Finally, the recovery of ectoine through bacterial bio-milking resulted in efficiencies of >70% without any negative impact of methanotrophic cell recycling to the bioreactors on CH biodegradation or ectoine synthesis.

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http://dx.doi.org/10.1016/j.watres.2023.120665DOI Listing

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