sp. nov., isolated from the fermented fruit of a medicinal plant.

Int J Syst Evol Microbiol

Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.

Published: October 2023

A facultative anaerobic and Gram-negative strain, designated RP14, was isolated from the fruit of fermented for 60 days at 25°C. Strain RP14 showed 98.0 % 16S rRNA similarity to IFO 15243, but in the phylogenetic tree, NIBRBAC000500504 was its closest neighbour. The average nucleotide identity and digital DNA-DNA hybridization values between strain RP14 and 15 genomes of type strains of , were 73.8-74.4% and 16.4-20.2 %, respectively, which were lower than the recommended thresholds for species delineation. The strain grew at 25-32°C (optimum, 28°C), at pH 7.0-12.0 (optimum, pH 9.0) and with 0-2% NaCl (optimum, 0 %; w/v). Cells of strain RP14 were catalase-positive, oxidase-negative, rod-shaped and formed yellow-coloured colonies. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major fatty acid were C, C cyclo 8 and summed feature 8 (C 7 and/or C 6). The DNA G+C content was 62.8 mol%. Based on polyphasic evidence, we propose sp. nov as a novel species within the genus The type strain is RP14 (=KACC 22720=TBRC 16341).

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Source
http://dx.doi.org/10.1099/ijsem.0.006086DOI Listing

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